purification, extraction and visualization of lipopolysaccharide of escherichia coli from urine samples of patients with urinary tract infection
نویسندگان
چکیده
conclusions our successful extraction of purified lps from e. coli isolates of uti patients’ urine samples can be an important step to understand the uti disease conditions. results the silver-stained gel demonstrated both smooth and rough type lps by showing trail-like band patterns with the presence and lacking o-antigen region, respectively. coomassie blue staining showed no band assuring the absence of any contaminating protein. patients and methods the e. coli strain was isolated from the urine samples of 10 patients with uti and then the antibiotic sensitivity pattern of the isolates was determined. the purification of lps was carried out using the hot aqueous-phenol method and separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis, which was directly stained using the modified silver staining method and coomassie blue. background urinary tract infection (uti) is one of the most common infectious diseases in bangladesh where escherichia coli is the prevalent organism and responsible for most of the infections. lipopolysaccharide (lps) is known to act as a major virulence factor of e. coli. objectives the present study aimed to purify, extract and visualize lps of e. coli clinical isolates from urine samples of patients with uti.
منابع مشابه
Comparative virulotyping and phylogenomics of Escherichia coli isolates from urine samples of men and women suffering urinary tract infections
Objective(s): Escherichia coli strains are common pathogens that can cause urinary tract infections (UTI). This study aimed to assess E. coli phylogroups and virulence types in male and female UTI patients.Materials and Methods: In the present study, 160 uropathogenic E. coli (UPEC) isolates (from both sexes) were assigned to phylogroups/types and some extraintestinal virulence factors were det...
متن کاملEvaluation of Quinolone Resistance in Escherichia coli Isolates Recovered from Urine and Feces of Patients with Acute or Recurrent Urinary Tract Infection
Introduction: Antibiotic resistance, especially in Gram-negative uropathogens such as Escherichia coli, is the main barrier to treat urinary tract infection (UTI). In recent years, the dramatically increased resistance of E. coli to quinolones, a group of widely used antibiotics, has become a significant concern. Methods: In this descriptive cross-sectional study, we collected 261 E. coli isola...
متن کاملResistance patterns of Escherichia coli causing urinary tract infection
Background: Urinary tract infection (UTI) is one of the most prevalent infectious diseases and Escherichia coli is its common cause. The aim of this study was to assess the resistance patterns of E.coli in urinary tract infections and to determine the susceptibility of E.coli to commonly used antimicrobials and also to evaluate the options for empirical treatment of UTI. Methods: This stud...
متن کاملBiochemical Detection of N-Acyl Homoserine Lactone from Biofilm-Forming Uropathogenic Escherichia coli Isolated from Urinary Tract Infection Samples
Background: N-Acyl homoserine lactone (AHL) is found to be the main component of quorum sensing (QS) in Gram-negative bacteria and plays an important role in biofilm formation. Little information is available regarding the role of AHL in biofilm formation in Escherichia coli (E. coli). The purpose of this investigation was to biochemically detect and characterize AHL activity in biofilm-forming...
متن کاملIdentification of extended-spectrum β-lactamase genes in Escherichia coli isolated from patients with urinary tract infection
Introduction: Recentlly, resistance to antibiotics has increased and antibiotic-resistant strains producing extended-spectrum beta-lactamases (ESBLs) have emerged among Enterobacteriaceae, mainly in Escherichia coli (E. coli). In this study we aimed to determine phenotypic and genotypic ESBL production in isolated E. coli from women with urinary tract infection (UTI). Materials and Methods: In...
متن کاملPrevalence of plasmid-encoded carbapenemases in multi-drug resistant Escherichia coli from patients with urinary tract infection in northern Iran
Objective(s): Resistance to carbapenems as the last line for controlling resistant bacteria is increasing due to production of carbapenemase. The aim of this study was to detect the plasmid-encoded carbapenemases using phenotypic methods and multiplex PCR among the multi-drug resistant (MDR) isolates from patients with urinary tract infection (UTI) in northern Iran.<br...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
avicenna journal of clinical microbiology and infectionجلد ۲، شماره ۴، صفحات ۰-۰
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023